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科普小课堂 | 辅酶 A 的色谱方法
人阅读 发布时间：2020-12-25 17:43
科普小课堂 | 辅酶 A 系列产品的色谱方法
辅酶 A（coenzyme A），是一种辅酶，值得注意的是其在合成和氧化脂肪酸的角色，和在三羧酸循环中氧化丙酮酸。所有基因组测序日期编码的酶，即利用辅酶A作为底物，并在4%左右的细胞酶中使用（或硫酯，例如乙酰-CoA）作为基材。在人类中，辅酶A生物合成需要半胱氨酸、泛酸和三磷酸腺苷（ATP）。主要参与脂肪酸以及丙酮酸的代谢。
关于ZL-23010 – ZL-23110 的辅酶A系列产品，国外建议如下：
辅酶A衍生物不建议使用普通的RP C18色谱柱进行分析，因这类化合物会粘附在色谱柱上，难以从色谱柱中洗脱出来。
色谱条件建议如下：
若您选取的是普通的C18色谱柱，溶剂A务必调整为0.2% 三.氟.乙.酸(TFA) 水溶液即每1L水中三.氟.乙.酸用量2mL (溶剂A)。
梯度洗脱 溶剂A：0.2％TFA水溶液 溶剂B：乙腈/甲醇
0 - 8 min A：B = 80：20
8 - 9 min B = 100%
9 - 10 min A：B = 80：20
国外关于该类产品的原文说明如下：
Please tell the customer he should read the Certificate of analysis with attention,
because the Coenzyme-A-Derivatives can NOT be tested with usual, ordinary RP C18 columns, because the compound is stick (adhere) on the column an it is NOT eluting from the column !!!
For getting elution, the customer need to use Chromolith Type columns with 0.1% Formic Acid in Water and starting HPLC-MS conditions with 10% Solvent B (Acetonitril or MeOH) and 90% Solvent A (Water with 0.1% Formic Acid).
In case of using common RP C18 columns, proceed as following: Use as Solvent A 0.2% TFA (Trifluoroacetic Acid - this is meaning 2ml TFA per Liter Water) and starting HPLC-MS conditions using 20% Solvent B (Acetonitril or MeOH) and 90% Solvent A (Water with 0.2% Trifluoroacetic Acid) than increase in 8 minutes the Solvent B to 100%, keep 1 minute at 100% and go back to 20% Solvent B in 1 Minute keeping this gradient for another minute.
辅酶 A（coenzyme A），是一种辅酶，值得注意的是其在合成和氧化脂肪酸的角色，和在三羧酸循环中氧化丙酮酸。所有基因组测序日期编码的酶，即利用辅酶A作为底物，并在4%左右的细胞酶中使用（或硫酯，例如乙酰-CoA）作为基材。在人类中，辅酶A生物合成需要半胱氨酸、泛酸和三磷酸腺苷（ATP）。主要参与脂肪酸以及丙酮酸的代谢。
关于ZL-23010 – ZL-23110 的辅酶A系列产品，国外建议如下：
辅酶A衍生物不建议使用普通的RP C18色谱柱进行分析，因这类化合物会粘附在色谱柱上，难以从色谱柱中洗脱出来。
色谱条件建议如下：
- Chromolith Type色谱柱
- 洗脱剂：10％溶剂B（乙腈/甲醇）+ 90％溶剂A（0.1％甲酸水溶液）
若您选取的是普通的C18色谱柱，溶剂A务必调整为0.2% 三.氟.乙.酸(TFA) 水溶液即每1L水中三.氟.乙.酸用量2mL (溶剂A)。
梯度洗脱 溶剂A：0.2％TFA水溶液 溶剂B：乙腈/甲醇
0 - 8 min A：B = 80：20
8 - 9 min B = 100%
9 - 10 min A：B = 80：20
国外关于该类产品的原文说明如下：
Please tell the customer he should read the Certificate of analysis with attention,
because the Coenzyme-A-Derivatives can NOT be tested with usual, ordinary RP C18 columns, because the compound is stick (adhere) on the column an it is NOT eluting from the column !!!
For getting elution, the customer need to use Chromolith Type columns with 0.1% Formic Acid in Water and starting HPLC-MS conditions with 10% Solvent B (Acetonitril or MeOH) and 90% Solvent A (Water with 0.1% Formic Acid).
In case of using common RP C18 columns, proceed as following: Use as Solvent A 0.2% TFA (Trifluoroacetic Acid - this is meaning 2ml TFA per Liter Water) and starting HPLC-MS conditions using 20% Solvent B (Acetonitril or MeOH) and 90% Solvent A (Water with 0.2% Trifluoroacetic Acid) than increase in 8 minutes the Solvent B to 100%, keep 1 minute at 100% and go back to 20% Solvent B in 1 Minute keeping this gradient for another minute.
| 货号 | 中文品名 | 英文品名 | CAS | 规格 |
| ZL-23014 | 巴豆酰辅酶 A 三锂盐 | Crotonoyl coenzyme A trilithium salt | N/A | 5mg |
| ZL-23018 | 3-羟基苯甲酰辅酶A | 3-Hydroxybenzoyl-CoA | N/A | 5mg |
| ZL-23019 | N-甲基氨茴酰辅酶A | N-Methylanthraniloyl-CoA | N/A | 5mg |
| ZL-23024 | 乙酰丙酰基辅酶A | Levulinyl-CoA | N/A | 5mg |
| ZL-23027 | 2-羟基肉桂酰辅酶A | 2-Hydroxycinnamoyl-CoA | N/A | 5mg |
| ZL-23029 | ß-苯并氨酰辅酶A | ß-Phenylalanoyl-CoA | N/A | 5mg |
| ZL-23034 | 巴豆酰辅酶 | Crotonyl-Coenzyme A | 992-67-6 | 15mg |
| ZL-23038 | 丁酰辅酶A | Butiryl-Coenzyme A | N/A | 15mg |
| ZL-23026 | 辅酶A钠盐 水合物 | Coenzyme A sodium salt hydrate/Coenzyme A | 55672-92-9/85-61-0 | 15mg |
| ZL-23032 | 乙酰辅酶A，锂盐 | Acetyl-Coenzyme A,Lithium Salt | 32140-51-5 (Salt) | 15mg |
| ZL-23035 | 异丁基辅酶A | Isobutyryl-Coenzyme A | N/A | 15mg |
| ZL-23039 | 丙二酰辅酶A | Malonyl-Coenzyme A | 524-14-1 | 15mg |
| ZL-23040 | 3-氧代己酰辅酶A | 3-oxohexanoyl Coenzyme A | N/A | 15mg |
| ZL-23041 | 2-乙基-ß-酮丁酰辅酶A钠盐 | 2-ethyl-ß-ketobutiryl Coenzyme A Na Salt | 15mg | |
| ZL-23042 | 3-羟基己酰辅酶A | 3-hydroxyhexanoyl Coenzyme A | N/A | 15mg |
| ZL-23043 | 4-羟基苯甲酰辅酶A | 4-hydroxybenzoyl Coenzyme A | 27718-41-8 | 15mg |
| ZL-23044 | 3-羟基苯甲酰辅酶A | 3-hydroxybenzoyl Coenzyme A | 52301-52-7 | 15mg |
| ZL-23045 | 邻香豆酰辅酶A | o-Coumaryl-Coenzyme A | N/A | 15mg |
| ZL-23046 | ß-苯丙氨酸辅酶A | ß-Phenylalanin Coenzyme A | N/A | 15mg |
| ZL-23047 | 3,4-二羟基肉桂酸辅酶A | 3,4-Dihydroxycinnamic Coenzyme A | N/A | 15mg |
| ZL-23048 | 二氢咖啡酰辅酶A | Dihydrocaffeoyl Coenzyme A | N/A | 15mg |
| ZL-23049 | 异黄酮酰辅酶A | Isoferuoyl-Coenzym A | N/A | 15mg |
| ZL-23050 | 月桂酰辅酶A（锂盐） | Lauroyl Coenzyme A (lithium salt) | 190063-12-8 | 15mg |
| ZL-23110 | ß-苯丙氨酸辅酶A | ß-Phenylalanin-Coenzyme A | N/A | 15mg |
| ZL-23015 | 对羟基苯丙基辅酶A | Phloretic-CoA | N/A | 15mg |
| ZL-23017 | 3-羟基苯甲酰辅酶A | 3-Hydroxy-Benzoyl-CoA | N/A | 15mg |
| ZL-23025 | 乙酰辅酶A | Acetyl Coenzyme A | 72-89-9 | 15mg |
| ZL-23028 | 丙烯酰辅酶A | Acrylyl-Coenzyme A | 5776-58-9 | 15mg |
| ZL-23036 | 对香豆酰辅酶A | p-Coumaryl-Coenzyme A | 119785-99-8 | 15mg |
| ZL-23037 | 对二氢香豆酰辅酶A | p-Dihydrocoumaroyl-Coenzyme A | N/A | 15mg |
| ZL-23053 | 肉桂酰辅酶A | Cinnamyl-Coenzyme A | N/A | 15mg |
| ZL-23055 | 苯甲酰辅酶A | Benzoyl-Coenzyme A | N/A | 15mg |
| ZL-23068 | 咖啡酰辅酶A | Caffeoyl-Coenzym A | N/A | 15mg |
| ZL-23071 | 阿魏酰辅酶A | Feruloyl-Coenzyme A | N/A | 15mg |
| ZL-23075 | N-Methylantranyl-辅酶A | N-Methylantranyl-Coenzyme A | N/A | 15mg |
| ZL-23077 | 2-戊.烯酰辅酶A | 2-pentenoyl-Coenzyme A | N/A | 15mg |
| ZL-23078 | 芥子辅酶A | Sinapyl-Coenzyme A | N/A | 15mg |
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